| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
14/04/2009 |
|
Data da última atualização: |
30/05/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
COELHO, M. R. R.; MARRIEL, I. E.; JENKINS, S. N.; LANYON, C. V.; SELDIN, L.; O'DONNELL, A. G. |
|
Afiliação: |
Márcia R. R. Coelho, UFRJ; IVANILDO EVODIO MARRIEL, CNPMS; Sasha N. Jenkins, Newcastle University; Clare V. Lanyon, Newcastle University; Lucy Seldin, UFRJ; Anthony G. O'Donnell, Newcastle University. |
|
Título: |
Molecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
Applied Soil Ecology, Amsterdam, v. 42, n. 1, p. 48-53, 2009. |
|
DOI: |
10.1016/j.apsoil.2009.01.010 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Denaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important factors influencing the structure and amount of diazotrophs in sorghum. MenosDenaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important... Mostrar Tudo |
|
Thesagro: |
Sorgo. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53597/1/Molecular-detection.pdf
|
|
Marc: |
LEADER 02260naa a2200205 a 4500
001 1491948
005 2018-05-30
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1016/j.apsoil.2009.01.010$2DOI
100 1 $aCOELHO, M. R. R.
245 $aMolecular detection and quantification of nifH gene sequences in the rhizosphere of sorghum (Sorghum bicolor) sown with two levels of nitrogen fertilizer.$h[electronic resource]
260 $c2009
520 $aDenaturing gradient gel electrophoresis (DGGE) and SYBR Green I quantitative real-time PCR (qPCR) approaches were used to assess respectively the molecular diversity and the quantity of the nifH gene sequences in the rhizospheres of two cultivars of sorghum sown in Cerrado soil with contrasting levels of nitrogen fertilizer. DGGE fingerprinting showed that for both cultivars the presumptive nitrogen-fixing populations in the rhizospheres were more diverse than in bulk soil. Sequencing of nifH gene fragments obtained from DGGE bands revealed that members of the order Rhizobiales were prevalent among the dominant diazotrophs. Discriminant analysis of DGGE profiles resulted into three groups formed by (i) cultivar BRS 308 sown with high level of nitrogen, (ii) cultivar BRS 308 sown with low level of nitrogen and cultivar BRS 310 sown either with low or high levels of nitrogen and (iii) bulk soil, showing that the nitrogen fertilization influenced the nifH gene sequence diversity only in the rhizosphere of cultivar BRS 308. When the quantity of the nifH gene sequences was determined by q-PCR, 2.4 x105 to 1.3 x 107 copies/g of soil were detected. The highest number of nifH gene copies was observed in the rhizosphere of cultivar BRS 308 treated with low amount of fertilizer, and a reduction in nifH density was observed in the rhizospheres of both sorghum cultivars when high levels of nitrogen were applied. Thus, both the amount of nitrogen fertilizer and the cultivar are important factors influencing the structure and amount of diazotrophs in sorghum.
650 $aSorgo
700 1 $aMARRIEL, I. E.
700 1 $aJENKINS, S. N.
700 1 $aLANYON, C. V.
700 1 $aSELDIN, L.
700 1 $aO'DONNELL, A. G.
773 $tApplied Soil Ecology, Amsterdam$gv. 42, n. 1, p. 48-53, 2009.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Clima Temperado. Para informações adicionais entre em contato com cpact.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Uva e Vinho. |
|
Data corrente: |
16/12/2014 |
|
Data da última atualização: |
17/12/2014 |
|
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
|
Autoria: |
MAY DE MIO, L. L.; GARRIDO, L. da R.; UENO, B.; FAJARDO, T. V. M. |
|
Afiliação: |
LOUISE LARISSA MAY DE MIO, UFPR; LUCAS DA RESSURREICAO GARRIDO, CNPUV; BERNARDO UENO, CPACT; THOR VINICIUS MARTINS FAJARDO, CNPUV. |
|
Título: |
Doenças da cultura do pessegueiro e métodos de controle. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
In: RASEIRA, M. do C. B.; PEREIRA, J. F. M.; CARVALHO, F. L. C. (Ed.). Pessegueiro. Brasília, DF: Embrapa, 2014. p. 355-432. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Doença bacteriana. |
|
Thesagro: |
Doença de planta; Doença fúngica; Pessego. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00613naa a2200193 a 4500
001 2002705
005 2014-12-17
008 2014 bl uuuu u00u1 u #d
100 1 $aMAY DE MIO, L. L.
245 $aDoenças da cultura do pessegueiro e métodos de controle.
260 $c2014
650 $aDoença de planta
650 $aDoença fúngica
650 $aPessego
653 $aDoença bacteriana
700 1 $aGARRIDO, L. da R.
700 1 $aUENO, B.
700 1 $aFAJARDO, T. V. M.
773 $tIn: RASEIRA, M. do C. B.; PEREIRA, J. F. M.; CARVALHO, F. L. C. (Ed.). Pessegueiro. Brasília, DF: Embrapa, 2014. p. 355-432.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Clima Temperado (CPACT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
